A page from the OpenDepot.org service

Jump to the start of the main contents

Himratul-Aznita, W. H. and Mohd-Al-Faisal, N. and Fathilah, A. R. (2011) Determination of the percentage inhibition of diameter growth (PIDG) of Piper betle crude aqueous extract against oral Candida species. Journal of Medicinal Plants Research, 5 (6). pp. 878-884. ISSN 1996-0875

[img] PDF
Restricted to Registered users only

Download (110Kb) | Request a copy
    [img] PDF
    Download (105Kb)

      Abstract

      Species within the genus Candida have been implicated in many fungal diseases such as candidiasis or thrush. The increasing clinical and microbiological resistance of Candida species towards several commonly prescribed antifungal agents however, has lead to the search for new active antifungal compounds from natural resources. This study was carried out to screen the susceptibility of the aqueous extract of Piper betle towards seven species of oral Candida. It was found that P. betle extract exhibited high antifungal activities towards Candida albicans, Candida tropicalis, Candida glabrata, Candida dubliniensis, Candida lusitaniae, Candida krusei and Candida parapsilosis. The minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) value of P. betle extract towards all Candida species was found to be similar (12 mg/ml) except towards C. albicans which has been shown to have MIC value of 12 mg/ml and slightly higher MFC value of 25 mg/ml. The recorded data on the growth responses of the species to various concentrations of the extract following a 24 h incubation period were analysed, using the percentage inhibition of diameter growth (PIDG) against chlorhexidine gluconate (CHX). The determination of PIDG values for C. albicans, C. tropicalis, C. lusitaniae, C. dubliniensis and C. glabrata has shown that the aqueous extract of P. betle outstrips the positive control used, that was 0.12 w/v chlorhexidine with PIDG values of more than 50 at P. betle concentration of 25 mg/ml. In contrast, PIDG for C. krusei and C. parapsilosis shows that at 25 mg/ml concentration of P. betle extract has little influence on growth inhibition compared to CHX. Thus, the results obtained have shown the potential use of P. betle extract as antifungal agent and thus significantly contribute to its antifungal development.

      Item Type: Article
      Additional Information: ISI Document Delivery No.: 780VT Times Cited: 0 Cited Reference Count: 39 Cited References: Devi KP, 2010, J ETHNOPHARMACOL, V130, P107, DOI 10.1016/j.jep.2010.04.025 Fathilah A R, 2009, Pak J Biol Sci, V12, P518, DOI 10.3923/pjbs.2009.518.521 Sharma S, 2009, ANTIMICROB AGENTS CH, V53, P216, DOI 10.1128/AAC.00045-08 BOKORBRATIC MB, 2008, P NAT SCI, V114, P69 Nalina T., 2007, American Journal of Biochemistry and Biotechnology, V3, P10 Ramage G, 2006, FEMS YEAST RES, V6, P979, DOI 10.1111/j.1567-1364.2006.00117.x He XY, 2006, MYCOSES, V49, P80, DOI 10.1111/j.1439-0507.2006.01189.x Kauffman Carol A, 2006, Proc Am Thorac Soc, V3, P35, DOI 10.1513/pats.200510-110JH FATHILAH AR, 2006, J ORAL SCI, V48, P71 Dasgupta N, 2004, FOOD CHEM, V88, P219, DOI 10.1016/j.foodchem.2004.01.036 Ramji N, 2002, J ETHNOPHARMACOL, V83, P149, DOI 10.1016/S0378-8741(02)00194-0 Friedman M, 2002, J FOOD PROTECT, V65, P1545 Espinel-Ingroff A, 2002, J CLIN MICROBIOL, V40, P3204, DOI 10.1128/JCM.40.9.3204-3208.2002 Chang MC, 2002, BRIT J PHARMACOL, V135, P619, DOI 10.1038/sj.bjp.0704492 Ong HC, 1999, FITOTERAPIA, V70, P502, DOI 10.1016/S0367-326X(99)00077-5 Barchiesi F, 1999, J ANTIMICROB CHEMOTH, V43, P295, DOI 10.1093/jac/43.2.295 Jorgensson JH, 1999, MANUAL CLIN MICROBIO, P1526 Hirasa K., 1998, SPICE SCI TECHNOLOGY COLEMAN D, 1997, ORAL DIS, V3, P96 VARIER PS, 1997, INDIAN MED PLANT, V4, P279 Nguyen MH, 1996, AM J MED, V100, P617 KIM JM, 1995, J AGR FOOD CHEM, V43, P2839, DOI 10.1021/jf00059a013 SIKKEMA J, 1995, MICROBIOL REV, V59, P201 Prabhu M. Sandhya, 1995, Indian Journal of Experimental Biology, V33, P752 CIANCIO SG, 1995, PERIODONTOL 2000, V8, P75, DOI 10.1111/j.1600-0757.1995.tb00046.x JUVEN BJ, 1994, J APPL BACTERIOL, V76, P626, DOI 10.1111/j.1365-2672.1994.tb01661.x ODDEN K, 1994, J ORAL PATHOL MED, V23, P178, DOI 10.1111/j.1600-0714.1994.tb01109.x POWDERLY WG, 1992, AIDS, V6, P604 SLOTS J, 1990, ORAL MICROBIOL IMMUN, V5, P305, DOI 10.1111/j.1399-302X.1990.tb00430.x ODDS FC, 1990, MYCOSES IN AIDS PATIENTS, P67 PFALLER MA, 1990, ANTIMICROB AGENTS CH, V39, P1 HEIMDAHL A, 1989, ORAL SURG ORAL MED O, V68, P711, DOI 10.1016/0030-4220(89)90160-6 HENDERSON DK, 1989, ARCH INTERN MED, V149, P2172, DOI 10.1001/archinte.149.10.2172 WALKER CB, 1988, J CLIN PERIODONTOL, V15, P499, DOI 10.1111/j.1600-051X.1988.tb01021.x SLOTS J, 1988, Oral Microbiology and Immunology, V3, P47, DOI 10.1111/j.1399-302X.1988.tb00080.x GONZALEZ S, 1987, J PERIODONTOL, V58, P119 RIMANDO A M, 1986, Archives of Pharmacal Research (Seoul), V9, P93, DOI 10.1007/BF02857217 BAUER AW, 1966, AM J CLIN PATHOL, V45, P493 Chopra RN, 1956, GLOSSARY INDIAN MED, P194 Himratul-Aznita, W. H. Mohd-Al-Faisal, N. Fathilah, A. R. University of MalayaRG095/09HTM This study was financially supported by the University of Malaya Research Grant, RG095/09HTM. Academic journals Victoria island
      Uncontrolled Keywords: Candida sp. oral Candida Piper betle antifungal activity percentage inhibition of diameter growth (PIDG) essential oil in-vitro antifungal agents periodontitis constituents infections membrane
      Subjects: Medicine and Dentistry
      Divisions: UNSPECIFIED
      Depositing User: Mr Ahmad Azwan Azman
      Date Deposited: 28 Jun 2012 02:01
      Last Modified: 28 Jun 2012 02:01
      URI: http://opendepot.org/id/eprint/1262

      Actions (login required)

      View Item