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Othman, N. and Mohamed, Z. and Verweij, J. J. and Huat, L. B. and Olivos-Garcia, A. and Yeng, C. and Noordin, R. (2010) Application of Real-Time Polymerase Chain Reaction in Detection of Entamoeba histolytica in Pus Aspirates of Liver Abscess Patients. Foodborne Pathogens and Disease, 7 (6). pp. 637-641. ISSN 1535-3141

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    Abstract

    Entamoeba histolytica is the second major cause of liver abscess disease in humans, particularly in developing countries. Recently, DNA molecular-based methods have been employed to enhance the detection of E. histolytica in either pus or stool specimens. In this study, the results of real-time polymerase chain reaction (PCR) to detect E. histolytica DNA in pus from liver abscess cases were compared with those of indirect hemagglutination assay on the corresponding serum samples. Bacterial cultures were also performed on the pus samples for the diagnosis of pyogenic liver abscess. The real-time PCR detected E. histolytica DNA in 23 of 30 (76.7) pus samples, when compared with 14 of 30 (46.7) serum samples in which anti-Entamoeba antibodies were detected by indirect hemagglutination assay and 4 of 30 (13.3) pus samples that showed bacterial infection by culture. The use of real-time PCR is a promising detection method for diagnosis and epidemiology assessment of amoebic liver abscess.

    Item Type: Article
    Additional Information: ISI Document Delivery No.: 605BJ Times Cited: 2 Cited Reference Count: 22 Cited References: Ahmad N, 2007, Bangladesh Med Res Counc Bull, V33, P13 Fotedar R, 2007, CLIN MICROBIOL REV, V20, P511, DOI 10.1128/CMR.00004-07 Hall T. A., 1999, NUCL ACIDS S SER, V41, P95 Haque Rashidul, 1998, Parasitology International, V47, P201, DOI 10.1016/S1383-5769(98)00020-8 Haque R, 2003, NEW ENGL J MED, V348, P1565, DOI 10.1056/NEJMra022710 Haque R, 2006, ARCH MED RES, V37, P273, DOI 10.1016/j.arcmed.2005.09.001 HAQUE R, 2000, J CLIN MICROBIOL, V38, P3255 Hira PR, 2001, AM J TROP MED HYG, V65, P341 Klein D, 2002, TRENDS MOL MED, V8, P257, DOI 10.1016/S1471-4914(02)02355-9 MOHAMMADI SS, 2006, J CLIN MICROBIOL, V44, P2258 Niesters HGM, 2002, J CLIN VIROL, V25, pS3 OBOYLE C, 1988, GUT, V42, P29 Parija SC, 2007, BMC MICROBIOL, V7, DOI 10.1186/1471-2180-7-41 Qvarnstrom Y, 2005, J CLIN MICROBIOL, V43, P5491, DOI 10.1128/JCM.43.11.5491-5497.2005 Rani R, 2006, AM J TROP MED HYG, V75, P880 Roy S, 2005, J CLIN MICROBIOL, V43, P2168, DOI 10.1128/JCM.43.5.2168-2172.2005 RUSTGI AK, 1989, MED CLIN N AM, V73, P847 Verweij JJ, 2003, TROP MED INT HEALTH, V8, P1153, DOI 10.1046/j.1360-2276.2003.01145.x Verweij JJ, 2004, J CLIN MICROBIOL, V42, P1220, DOI 10.1128/JCM.42.3.1220-1223.2004 World Health Organization, 1997, WKLY EPIDEMIOL REC, V72, P97 Zeehaida M, 2008, TROP BIOMED, V25, P209 Zengzhu G, 1999, J CLIN MICROBIOL, V37, P3034 Othman, Nurulhasanah Mohamed, Zeehaida Verweij, Jaco J. Huat, Lim Boon Olivos-Garcia, Alfonso Yeng, Chen Noordin, Rahmah Malaysian Ministry of Higher Education (FRGS)203/CIPPM/6711122; university's Vice Chancellor Award This study was funded by a research grant from the Malaysian Ministry of Higher Education (FRGS grant no. 203/CIPPM/6711122). The first author received financial support through the university's Vice Chancellor Award. Mary ann liebert inc New rochelle
    Uncontrolled Keywords: diagnosis amebiasis pcr antigen samples assay ala
    Subjects: Medicine and Dentistry > Clinical Dentistry
    Divisions: UNSPECIFIED
    Depositing User: Mr Ahmad Azwan Azman
    Date Deposited: 15 Oct 2012 10:36
    Last Modified: 15 Oct 2012 10:36
    URI: http://opendepot.org/id/eprint/1434

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