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Torey, A. and Sasidharan, S. and Yeng, C. and Latha, L. Y. (2010) Standardization of Cassia spectabilis with Respect to Authenticity, Assay and Chemical Constituent Analysis. Molecules, 15 (5). pp. 3411-3420. ISSN 1420-3049

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      Abstract

      Quality control standardizations of the various medicinal plants used in traditional medicine is becoming more important today in view of the commercialization of formulations based on these plants. An attempt at standardization of Cassia spectabilis leaf has been carried out with respect to authenticity, assay and chemical constituent analysis. The authentication involved many parameters, including gross morphology, microscopy of the leaves and functional group analysis by Fourier Transform Infrared (FTIR) spectroscopy. The assay part of standardization involved determination of the minimum inhibitory concentration (MIC) of the extract which could help assess the chemical effects and establish curative values. The MIC of the C. spectabilis leaf extracts was investigated using the Broth Dilution Method. The extracts showed a MIC value of 6.25 mg/mL, independent of the extraction time. The chemical constituent aspect of standardization involves quantification of the main chemical components in C. spectabilis. The GCMS method used for quantification of 2,4-(1H,3H)-pyrimidinedione in the extract was rapid, accurate, precise, linear (R(2) = 0.8685), rugged and robust. Hence this method was suitable for quantification of this component in C. spectabilis. The standardization of C. spectabilis is needed to facilitate marketing of medicinal plants, with a view to promoting the export of valuable Malaysian Traditional Medicinal plants such as C. spectabilis.

      Item Type: Article
      Additional Information: ISI Document Delivery No.: 601YY Times Cited: 2 Cited Reference Count: 15 Cited References: Brain KR, 1975, PRACTICAL EVALUATION, P81 EVANS WC, 1996, TREASE EVANS PHARMAC, P545 GAEDCKE F, 2003, HERBAL MED PRODUCTS, P37 Lazarowych NJ, 1998, DRUG INF J, V32, P497 LORENZI H, 2002, PLANTAS MED BRASIL N, P291 National Committee for Clinical Laboratory Standards, 2002, M100S12 NCCLS Sangeetha S.ZurainiZ, 2008, INT J NAT ENG SCI, V2, P111 Sangetha S, 2008, Nihon Ishinkin Gakkai Zasshi, V49, P299, DOI 10.3314/jjmm.49.299 SANGETHA S, 2008, ASIAN J PHARM CLIN R, V1, P17 Sasidharan S, 2009, SAINS MALAYS, V38, P935 Soetardjo Surjani Jr, 2007, Malays J Nutr, V13, P81 Viegas C, 2004, J NAT PROD, V67, P908, DOI 10.1021/np0303963 World Health Organization, 1998, Quality controlmethods formedicinal plants Yadav NP, 2009, RECENT PROG MED PLAN, V24, P25 Yadav N. P., 2008, International Journal of Integrative Biology, V2, P195 Torey, Angeline Sasidharan, Sreenivasan Yeng, Chen Latha, Lachimanan Yoga University Sains Malaysia304/CIPPM/639040; Institute for Postgraduate Studies (IPS) of Universiti Sains Malaysia This project was funded by USM Short Term Grants (304/CIPPM/639040) from University Sains Malaysia. Angeline Torey is supported by the Universiti Sains Malaysia Fellowship Scheme from Institute for Postgraduate Studies (IPS) of Universiti Sains Malaysia. Molecular diversity preservation international-mdpi Basel
      Uncontrolled Keywords: Cassia spectabilis standardization microscopy medicinal plants
      Subjects: Medicine and Dentistry > Clinical Dentistry
      Divisions: UNSPECIFIED
      Depositing User: Mr Ahmad Azwan Azman
      Date Deposited: 16 Oct 2012 02:30
      Last Modified: 16 Oct 2012 02:30
      URI: http://opendepot.org/id/eprint/1438

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