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Yeng, C. and Osman, E. and Mohamed, Z. and Noordin, R. (2010) Detection of immunogenic parasite and host-specific proteins in the sera of active and chronic individuals infected with Toxoplasma gondii. Electrophoresis, 31 (23-24). pp. 3843-3849. ISSN 0173-0835

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    Abstract

    Toxoplasma gondii infection in pregnant women may result in abortion and foetal abnormalities, and may be life-threatening in immunocompromised hosts. To identify the potential infection markers of this disease, 2-DE and Western blot methods were employed to study the parasite circulating antigens and host-specific proteins in the sera of T. gondii-infected individuals. The comparisons were made between serum protein profiles of infected (n = 31) and normal (n = 10) subjects. Antigenic proteins were identified by immunoblotting using pooled sera and monoclonal anti-human IgM-HRP. Selected protein spots were characterised using mass spectrometry. Prominent differences were observed when serum samples of T. gondii-infected individuals and normal controls were compared. A significant up-regulation of host-specific proteins, alpha(2)-HS glycoprotein and alpha(1)-B glycoprotein, was also observed in the silver-stained gels of both active and chronic infections. However, only alpha(2)-HS glycoprotein and alpha(1)-B glycoprotein in the active infection showed immunoreactivity in Western blots. In addition, three spots of T. gondii proteins were detected, namely (i) hypothetical protein chrXII: 3984434-3 TGME 49, (ii) dual specificity protein phosphatase, catalytic domain TGME 49 and (iii) NADPH-cytochrome p450 reductase TGME 49. Thus, 2-DE approach followed by Western blotting has enabled the identification of five potential infection markers for the diagnosis of toxoplasmosis: three are parasite-specific proteins and two are host-specific proteins.

    Item Type: Article
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    Uncontrolled Keywords: 2-DE Host-specific protein Immunoblot Parasite protein Toxoplasma gondii adhesive protein hepatitis-c identification antigens fetuin inflammation expression proteomics diagnosis biomarker
    Subjects: Medicine and Dentistry > Clinical Dentistry
    Divisions: UNSPECIFIED
    Depositing User: Mr Ahmad Azwan Azman
    Date Deposited: 16 Oct 2012 02:33
    Last Modified: 16 Oct 2012 02:33
    URI: http://opendepot.org/id/eprint/1439

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