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Di Rocco, Giuliana and Gentile, Antonietta and Antonini, Annalisa and Ceradini, Francesca and Wu, Joseph C and Capogrossi, Maurizio C and Toietta, Gabriele (2011) Enhanced Healing of DiabeticWounds by Topical Administration of Adipose Tissue-Derived Stromal Cells Overexpressing Stromal-Derived Factor-1: Biodistribution and Engraftment Analysis by Bioluminescent Imaging. Stem Cells International, 2011. ISSN 1687-966X

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    Abstract

    Chronic ulcers represent a major health problem in diabetic patients resulting in pain and discomfort. Conventional therapy does not guarantee adequate wound repair. In diabetes, impaired healing is partly due to poor endothelial progenitor cells mobilisation and homing, with altered levels of the chemokine stromal-derived factor-1 (SDF-1) at the wound site. Adipose tissue-associated stromal cells (AT-SCs) can provide an accessible source of progenitor cells secreting proangiogenic factors and differentiating into endothelial-like cells. We demonstrated that topical administration of AT-SCs genetically modified ex vivo to overexpress SDF-1, promotes wound healing into diabetic mice. In particular, by in vivo bioluminescent imaging analysis, we monitored biodistribution and survival after transplantation of luciferase-expressing cells. In conclusion, this study indicates the therapeutic potential of AT-SCs administration in wound healing, through cell differentiation, enhanced cellular recruitment at the wound site, and paracrine effects associated with local growth-factors production.

    Item Type: Article
    Uncontrolled Keywords: wound healing; cell transplantation; diabetes; tissue regeneration; regenerative medicine; adipose tissue-derived stromal and vascular cells; SDF-1; cell engraftment
    Subjects: Medicine and Dentistry > Pre-clinical Medicine
    Biological Sciences
    Divisions: UNSPECIFIED
    Depositing User: Dr Gabriele Toietta
    Date Deposited: 30 Nov 2011 09:39
    Last Modified: 15 Dec 2016 10:10
    URI: http://opendepot.org/id/eprint/541

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