Govindasamy, V. and Ronald, V. S. and Abdullah, A. N. B. and Ganesan Nathan, K. R. and Aziz, Z. A. C. A. and Abdullah, M. and Zain, R. B. and Kasim, N. H. A. and Musa, S. and Bhonde, R. R. (2011) Human platelet lysate permits scale-up of dental pulp stromal cells for clinical applications. Cytotherapy, 13 (10). pp. 1221-1233. ISSN 1465-3249Full text not available from this repository. (Request a copy)
Background aims. Dental pulp stromal cells (DPSC) are considered to be a promising source of stem cells in the field of regenerative therapy. However, the usage of DPSC in transplantation requires large-scale expansion to cater for the need for clinical quantity without compromising current good manufacturing practice (cGMP). Existing protocols for cell culturing make use of fetal bovine serum (FBS) as a nutritional supplement. Unfortunately, FBS is an undesirable additive to cells because it carries the risk of transmitting viral and prion diseases. Therefore, the present study was undertaken to examine the efficacy of human platelet lysate (HPL) as a substitute for FBS in a large-scale set-up. Methods. We expanded the DPSC in Dulbecco's modified Eagle's mediumknock-out (DMEM-KO) with either 10 FBS or 10 HPL, and studied the characteristics of DPSC at pre- (T25 culture flask) and post- (5-STACK chamber) large-scale expansion in terms of their identity, quality, functionality, molecular signatures and cytogenetic stability. Results. In both pre- and post-large-scale expansion, DPSC expanded in HPL showed extensive proliferation of cells (c. 2-fold) compared with FBS; the purity, immune phenotype, colony-forming unit potential and differentiation were comparable. Furthermore, to understand the gene expression profiling, the transcriptomes and cytogenetics of DPSC expanded under HPL and FBS were compared, revealing similar expression profiles. Conclusions. We present a highly economized expansion of DPSC in HPL, yielding double the amount of cells while retaining their basic characteristics during a shorter time period under cGMP conditions, making it suitable for therapeutic applications.
|Uncontrolled Keywords:||dental pulp stromal cells fetal bovine serum human platelet lysate large-scale expansion regenerative medicine bovine serum albumin cell surface marker transcriptome animal cell article cell culture cell function cell growth cell isolation cell lineage cell lysate cell proliferation cell structure clinical practice colony forming unit controlled study culture optimization culture technique cytogenetics dental pulp stromal cell fetal calf serum gene expression profiling good manufacturing practice human human cell human tissue immunophenotyping in vitro study nonhuman osteoblast priority journal quality control stem cell expansion stroma cell thrombocyte tooth pulp|
|Subjects:||Medicine and Dentistry|
|Depositing User:||Prof. Dr. Rosnah Mohd Zain|
|Date Deposited:||03 Feb 2012 01:51|
|Last Modified:||03 Feb 2012 01:51|
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